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Related post: PI: R. E. Race OTHER: B. Chesebro M. E. Bloom Veterinary Officer Chief Medical Officer LPVD NIAID LPVD NIAID LPVD NIAID COOPERATING UNITS (if any) None LAB/BRANCH Laboratory of Persistent Viral Diseases, Hamilton, MT 59840 INSTITUTE AND LOCATION NTAin, NTH, Refhesda, MP 20205 TOTAL MANYEARS: PROFESSIONAL! .3_ OTHER: CHECK Cheap Zenegra APPROPRIATE BOX(ES) D (a) HUMAN SUBJECTS Q (al) MINORS D (a2) INTERVIEWS n (b) HUMAN TISSUES (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) The goal of this project is to define Zenegra Online immune mechanisms and viral character - istics important in the pathogenesis of Aleutian disease (AD). Monoclonal antibodies were used to study antigenic differences among ADV isolates. Antibodies with three predominant patterns of reactivity have been identified. 1) those that react with viral antigens extracted from tissues of infected mink, 2) those that react with viral antigens purified from ADV infected cell cultures, and 3) those that react with virus from either source. Antibodies recognizing viral antigens purified from ADV infected cell cultures recognize the major viral structural proteins of ADV while the viral associated antigen recognized by monoclonal antibodies reacting with tissue extract has not been determined. These Buy Zenegra reagents allow more precise characterization of ADV isolates than has been" possible previously. We are also using them Zenegra Uk as probes to localize viral antigen in tissues of infected mink. Spleen, liver, lymph node and kidney from infected mink have been examined so far but as yet no viral antigen has been demonstrated. Techniques will be modified and further attempts made in this regard. Additional monoclonal antibodies will also be used and new ones developed. 28-14 PHS-6040 ~ ' (Rev. 2-81) SMITHSONIAN SCIENCE INFORMATION EXCHANGE PROJECT NUMBER (Oo NOT use this space) U.S. DEPARTMENT OF HEALTH AND HUMAN Zenegra 50 SERVICES PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI Zenegra 100 00265-01 LPVD PERIOD COVERED October 1, 1981 to September 30, 1982 TITLE OF PROJECT (80 characters or less) Immunobiology of Scrapie Zenegra 100mg Virus Infection NAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER PROFESSIONAL PERSONNEL ENGAGED ON THE PROJECT PI: R. E. Race OTHER: B. Chesebro Veterinary Officer Chief LPVD NIAID LPVD NIAID COOPERATING UNITS (If any) None LAB/BRANCH Laboratory of Persistent Viral Diseases, Hamilton, MT 59840 SECTION INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MD 20205 TOTAL MANYEARS: .5 PROFESSIONAL: .5 CHECK APPROPRIATE BOX(ES) D (a) HUMAN SUBJECTS D (al) MINORS □ (a2) INTERVIEWS D (b) HUMAN TISSUES (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) The immediate goal of this project is to establish high titered scrapie infected tissue culture cell lines. Scrapie is a naturally occurring spongiform enceph- alopathy of sheep and goats which causes Zenegra 100 Mg clinical and pathological changes similar to those of Creutzfeldt- Jakob and Kuru diseases of man. Scrapie grows to high titer in mouse lymphoid tissue and brain but has never been passaged at high titer in any in vitro system. Adaptation of the agent to cell culture will permit detailed characterization of the agent(s) and provide a system for better definition of the pathogenesis of these diseases. We have utilized two approaches designed to provide infected in vitro cell lines. In one approach, scrapie infected mice are injected with one of several splenotropic tumor cell
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